Analysis of Protein Glycosylation by Mass Spectrometry.
Protein glycosylation involves combining carbohydrates and proteins to form glycoproteins .scientist have used this process in the investigation and research of various diseases and biopharmaceutical research. To analysis glycosylated proteins biomedical researchers use mass spectrometry method. Mass spectrometry uses the following procedures depending on the kit used to prepare samples for protein glycosylation analysis. It bases its process on the release of N glycans.to deglycosylate a sample use the following kits
Gly-X 2-AB Express kit
To prepare samples for research; the kit has a novel in-solution to help in enzymatic protein glycosylation. N glycan released is then Lebed with 2-AB dye. This kit is simple to use; it offers rapid and suitable procedure of protein glycosylation analysis using mass spectrometry. The final step involves washing the samples to remove compound that may hinder the experiment.
Gly-X instant pc dye kit
Gly -x pc kit uses a novel in-solutions to enzymatically deglycosylated a sample of experiments, N glycan released labelled with instant pc dye. The sample is then cleaned to remove excess stain and other compounds that may interfere with the analysis. Instant pc dye provides unmarked fluorescent brightness and mass spectrometry signal which provides a single labelling method deployed across several glycan protein analysis.
Glycan analysis system
It uses capillary electrophoresis with gel cartridge. The capillary appears on the cartridge that contains gel matrix. You can easily replace the matrix at the uppermost part the reservoir cartridge between samples runs. The reagents deglycosylate purified samples, and they remain inside reagents for 5 minutes to digest. You can instantly LaBelle the released glycan using glycan -Q instant dye. You can as well use 96 well plate and vacuum manifold during the experiment.
The first sample produces N glycan after 1hr and second sample that employs 96 plates after 6 hrs the Gly -Q platform is preferred for most glycan profiling.